Ge 71-5000-15 AD Manuel d'utilisateur

Instructions 71-5000-15 AD Pre-activated mediaGE HealthcareCNBr-activatedSepharose 4 Fast FlowIntroductionThe preparation and use of affinity chromat

p. 10 5. Cleaning, Sanitization and StorageFor best performance of coupled CNBr-activated Sepharose 4 Fast Flow over a long working life, follow th

p. 11 or Wash the column with 3– column volumes of 70% ethanol. Wash immediately with at least 5 column volumes of sterile filtered binding bu

BioProcess, BPG, CHROMAFLOW, Drop Design, Sepharose, and Tricorn are trademarks of GE Healthcare companies.GE, imagination at work and GE monogram are

p. 2 1. Product descriptionCNBr-activated Sepharose 4 Fast Flow is a bead-formed, highly cross-linked pre-activated matrix produced by reacting Sep

p. 3Table 1. Medium characteristics.Mean particle size 90 μm Particle size range 45–165 μm Bead structure Highly cross-linked 4% agarose

p. 4 In order to retain maximum binding capacity of CNBr-activated Sepharose 4 Fast Flow prior to coupling the ligand, use cold (0–4 °C) solutions

p. 55. Wash away exess ligand with at least 5 medium volumes of coupling buffer.6. After coupling, non-reacted groups on the medium should be b

p. 6 Large scale columns• BPGTM variable bed, glass columns. Inner diameters from 100–450 mm, bed volumes from 2.4–131 litres; bed height max 83 cm

p. 77. When the bed has stabilized, close the bottom outlet and stop the pump.8. If using a packing reservoir, disconnect the reservoir and fit

p. 8 The calculated plate number will vary depending on the test conditions and it should therefore be used as a reference value only. It is also i

p. 9The reduced plate height is calculated: HETP/dwhere d is the diameter of the bead. As a guideline, a value of <3 is normally acceptable.The